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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP54059PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP54059PU-N, RRID:AB_11146131
- Product name
- anti ST8SIA4 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 193~222 amino acids from the Central region of human ST8SIA4
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- LEFT: Western blot analysis of ST8SIA4 Antibody (Center) (Cat. #AP54059PU-N) in HL-60 cell line lysates (35µg/lane). ST8SIA4 (arrow) was detected using the purified Pab. RIGHT: Western blot analysis of ST8SIA4 Antibody (Center) (Cat. #AP54059PU-N) in mouse spleen tissue lysates (35µg/lane). ST8SIA4 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- ST8SIA4 Antibody (Center) (Cat. #AP54059PU-N) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ST8SIA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- ST8SIA4 Antibody (Center) (Cat. #AP54059PU-N) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
