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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN954963 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 4 (ST8SIA4) (AA 193-222), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 193~222 amino acids from the Central region of human ST8SIA4
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- AA 193-222,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references The ERAP2 gene is associated with preeclampsia in Australian and Norwegian populations.
Identification of sequences in the polysialyltransferases ST8Sia II and ST8Sia IV that are required for the protein-specific polysialylation of the neural cell adhesion molecule, NCAM.
Johnson MP, Roten LT, Dyer TD, East CE, Forsmo S, Blangero J, Brennecke SP, Austgulen R, Moses EK
Human genetics 2009 Nov;126(5):655-66
Human genetics 2009 Nov;126(5):655-66
Identification of sequences in the polysialyltransferases ST8Sia II and ST8Sia IV that are required for the protein-specific polysialylation of the neural cell adhesion molecule, NCAM.
Foley DA, Swartzentruber KG, Colley KJ
The Journal of biological chemistry 2009 Jun 5;284(23):15505-16
The Journal of biological chemistry 2009 Jun 5;284(23):15505-16
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- LEFT: Western blot analysis of ST8SIA4 Antibody (Center) (Cat. #AP54059PU-N) in HL-60 cell line lysates (35μg/lane). ST8SIA4 (arrow) was detected using the purified Pab. RIGHT: Western blot analysis of ST8SIA4 Antibody (Center) (Cat. #AP54059PU-N) in mouse spleen tissue lysates (35μg/lane). ST8SIA4 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ST8SIA4 Antibody (Center) (Cat. #AP54059PU-N) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ST8SIA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ST8SIA4 Antibody (Center) (Cat. #AP54059PU-N) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
