Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [6]
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Validation data
Reference
Comment
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- Product number
- NB100-2269 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2269, RRID:AB_2182046
- Product name
- Rabbit Polyclonal RPL7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references JUND regulates pancreatic β cell survival during metabolic stress.
MTHFSD and DDX58 are novel RNA-binding proteins abnormally regulated in amyotrophic lateral sclerosis.
CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins.
A novel function for the survival motoneuron protein as a translational regulator.
Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
Hematopoietic cell kinase associates with the 40S ribosomal subunit and mediates the ribotoxic stress response to deoxynivalenol in mononuclear phagocytes.
Rng3, a member of the UCS family of myosin co-chaperones, associates with myosin heavy chains cotranslationally.
Good AL, Cannon CE, Haemmerle MW, Yang J, Stanescu DE, Doliba NM, Birnbaum MJ, Stoffers DA
Molecular metabolism 2019 Jul;25:95-106
Molecular metabolism 2019 Jul;25:95-106
MTHFSD and DDX58 are novel RNA-binding proteins abnormally regulated in amyotrophic lateral sclerosis.
MacNair L, Xiao S, Miletic D, Ghani M, Julien JP, Keith J, Zinman L, Rogaeva E, Robertson J
Brain : a journal of neurology 2016 Jan;139(Pt 1):86-100
Brain : a journal of neurology 2016 Jan;139(Pt 1):86-100
CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins.
Gao J, Schatton D, Martinelli P, Hansen H, Pla-Martin D, Barth E, Becker C, Altmueller J, Frommolt P, Sardiello M, Rugarli EI
The Journal of cell biology 2014 Oct 27;207(2):213-23
The Journal of cell biology 2014 Oct 27;207(2):213-23
A novel function for the survival motoneuron protein as a translational regulator.
Sanchez G, Dury AY, Murray LM, Biondi O, Tadesse H, El Fatimy R, Kothary R, Charbonnier F, Khandjian EW, Côté J
Human molecular genetics 2013 Feb 15;22(4):668-84
Human molecular genetics 2013 Feb 15;22(4):668-84
Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
El Fatimy R, Tremblay S, Dury AY, Solomon S, De Koninck P, Schrader JW, Khandjian EW
PloS one 2012;7(6):e39338
PloS one 2012;7(6):e39338
Hematopoietic cell kinase associates with the 40S ribosomal subunit and mediates the ribotoxic stress response to deoxynivalenol in mononuclear phagocytes.
Bae H, Gray JS, Li M, Vines L, Kim J, Pestka JJ
Toxicological sciences : an official journal of the Society of Toxicology 2010 Jun;115(2):444-52
Toxicological sciences : an official journal of the Society of Toxicology 2010 Jun;115(2):444-52
Rng3, a member of the UCS family of myosin co-chaperones, associates with myosin heavy chains cotranslationally.
Amorim MJ, Mata J
EMBO reports 2009 Feb;10(2):186-91
EMBO reports 2009 Feb;10(2):186-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPL7 Antibody [NB100-2269] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells Antibody: Affinity purified rabbit anti-RPL7 antibody used for WB at 0.1ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPL7 Antibody [NB100-2269] - Detection of Human and Mouse RPL7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL7 antibody NB100-2269 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPL7 Antibody [NB100-2269] - Detection of human and mouse RPL7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TMCK-1, and mouse NIH 3T3 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-RPL7 antibody NB100-2269 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RPL7 Antibody [NB100-2269] - Detection of human RPL7 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-RPL7 antibody NB100-2269(lot NB100-2269-3) used for IP at 6 ug per reaction. RPL7 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2269-2). For blotting immunoprecipitated RPL7, NB100-2269 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPL7 Antibody [NB100-2269] - Human lung carcinoma and bronchioalveolar carcinoma pleural effusion.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPL7 Antibody [NB100-2269] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-RPL7 used at a dilution of 1:200 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RPL7 Antibody [NB100-2269] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-RPL7 used at a dilution of 1:200 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RPL7 Antibody [NB100-2269] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPL7 used at a dilution of 1:200 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPL7 Antibody [NB100-2269] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPL7 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPL7 Antibody [NB100-2269] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-RPL7 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB