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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB7527 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Angiopoietin-like Protein 6/ANGPTL6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Angiopoietin-like Protein 6/ANGPTL6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Angiopoietin-like 6 or recombinant mouse Angiopoietin-like 2 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q8ROZ6- Isotype
- IgG
- Antibody clone number
- 766544
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Angiopoietin-like Protein 6/ANGPTL6 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Angiopoietin-like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB7527) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-like Protein 6/ANGPTL6 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Angiopoietin-like Protein 6/ANGPTL6 in BaF3 Mouse Cell Line. Angiopoietin-like Protein 6/ANGPTL6 was detected in immersion fixed BaF3 mouse pro-B cell line using Rat Anti-Mouse Angiopoietin-like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB7527) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
