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LearnNBP2-32276
antibody from Novus Biologicals
Targeting: HM13
dJ324O17.1, H13, IMP1, IMPAS, PSENL3, PSL3, SPP, SPPL1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP2-32276 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Signal Peptide Peptidase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Signal Peptide Peptidase Antibody [NBP2-32276] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SPP antibody NBP2-32276 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Signal Peptide Peptidase Antibody [NBP2-32276] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-SPP antibody NBP2-32276 used for IP at 6 ug per reaction. SPP was also immunoprecipitated by rabbit anti-SPP antibody BL16569. For blotting immunoprecipitated SPP, NBP2-32276 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
