20416-1-AP

antibody from Invitrogen Antibodies

Targeting: HM13 dJ324O17.1, H13, IMP1, IMPAS, PSENL3, PSL3, SPP, SPPL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20416-1-AP

Provider

Invitrogen Antibodies

Product name

SPP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: VLGILALSH TISPFMNKFF PASFPNRQYQ LLFTQGSGEN KEEIINYEFD TKDLVCLGLS SIVGVWYLLR KHWIANNLFG LAFSLNGVEL LHLNNVSTGC ILLGGLFIYD V (111-220 aa encoded by BC008938)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

U2OS cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 40x lens).