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LearnMA5-31624
antibody from Invitrogen Antibodies
Targeting: KDM1B
AOF1, bA204B7.3, C6orf193, dJ298J15.2, FLJ33898, FLJ34109, FLJ43328, LSD2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-31624 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KDM1B Monoclonal Antibody (5E10C1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5E10C1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KDM1B in human AOF1 recombinant protein. Sample was incubated with KDM1B monoclonal antibody (Product # MA5-31624) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KDM1B in HEK293 (1) and AOF1 cell lysate. Samples were incubated with KDM1B monoclonal antibody (Product # MA5-31624) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of KDM1B in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with KDM1B monoclonal antibody (Product # MA5-31624) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of KDM1B in HeLa cells. Sample was incubated with KDM1B monoclonal antibody (Product # MA5-31624) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KDM1B in paraffin-embedded esophageal cancer tissue. Sample was incubated with KDM1B monoclonal antibody (Product # MA5-31624) using a dilution of 1:200-1:1000 followed by DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KDM1B in paraffin-embedded rectum cancer tissue. Sample was incubated with KDM1B monoclonal antibody (Product # MA5-31624) using a dilution of 1:200-1:1000 followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of KDM1B in HeLa cells (green). Sample was incubated with KDM1B monoclonal antibody (Product # MA5-31624) using a dilution of 1:200-1:400 followed by negative control (red).
