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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AAR-003-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenosine A2B Receptor (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.75 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HL-60 (lanes 1 and 2) cell line, rat brain (lanes 3 and 4) and mouse brain (lanes 5 and 6) lysates: - 1,3,5. Anti-AdenosineA2BReceptor (extracellular) Antibody (#AAR-003), (1:200).2,4,6. Anti-Adenosine A2BReceptor (extracellular) Antibody , preincubated with Adenosine A2BReceptor (extracellular) Blocking Peptide (#BLP-AR003).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of AdenosineA2BReceptorin rat PC12 cells - Cell surface detection of AdenosineA2BReceptor in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-AdenosineA2BReceptor (extracellular) Antibody (#AAR-003), (1:100) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . B. Merge of A and live view of the cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of AdenosineA2BReceptor in rat lung - Immunohistochemical staining of rat lung paraffin embedded sections using Anti-AdenosineA2BReceptor (extracellular) Antibody (#AAR-003), (1:50). Staining is present in the respiratory epithelium of the bronchiole (Br) as well as in the pneumonocytes of the alveolar wall (alveoli, (A). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the counterstain.
