Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Immunohistochemistry [4]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BSM-54101R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Id1 Monoclonal Antibody (5F1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody is specific for E-cadherin but does show some minor cross-reactivity with P-cadherin.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 5F1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1:293T Cells; Probed with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1:293T Cells; Probed with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF (ICC) staining with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF (ICC) staining with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF (ICC) staining with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF (ICC) staining with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells with Id1 (5F1) Monoclonal Antibody (bsm-54101R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
