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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52144PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52144PU-N, RRID:AB_11147357
- Product name
- anti ID1 / BHLHB24 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 73-101 amino acids from the Central region of Human ID1 / BHLHB24
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ID1 / BHLHB24 Antibody (Center) Cat.-No AP52144PU-N in mouse heart tissue lysates (35ug/lane). This demonstrates the ID1(PEI 1:100) antibody detected the ID1 protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ID1 / BHLHB24 Antibody (Center) Cat.-No AP52144PU-N in U251 cell line lysates (35ug/lane). This demonstrates the ID1 antibody detected the ID1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ID1 / BHLHB24 Antibody (Center) Cat.-No AP52144PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue reacted with ID1 / BHLHB24 Antibody (Center) Cat.-No AP52144PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of U251 cells using ID1 / BHLHB24 Antibody (Center) Cat.-No AP52144PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
