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LearnH00084701-M01
antibody from Novus Biologicals
Targeting: COX4I2
COX4-2, COX4B, COX4L2, COXIV-2, dJ857M17.2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00084701-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00084701-M01, RRID:AB_2085406
- Product name
- Mouse Monoclonal COX IV Isoform 2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. COX4I2 - cytochrome c oxidase subunit IV isoform 2 (lung)
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references From clinic to mechanism: Proteomics-based assessment of angiogenesis in adrenal pheochromocytoma.
The cellular stress proteins CHCHD10 and MNRR1 (CHCHD2): Partners in mitochondrial and nuclear function and dysfunction.
Control of human energy expenditure by cytochrome c oxidase subunit IV-2.
Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice.
Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2.
Differential regulation of HIF-mediated pathways increases mitochondrial metabolism and ATP production in hypoxic osteoclasts.
Cytochrome c oxidase subunit 4 isoform 2-knockout mice show reduced enzyme activity, airway hyporeactivity, and lung pathology.
Sex- and brain region-specific role of cytochrome c oxidase in 1-methyl-4-phenylpyridinium-mediated astrocyte vulnerability.
Brain region specificity of 3-nitropropionic acid-induced vulnerability of neurons involves cytochrome c oxidase.
Brain region-specific vulnerability of astrocytes in response to 3-nitropropionic acid is mediated by cytochrome c oxidase isoform expression.
Sun F, Zhuo R, Ma W, Yang D, Su T, Ye L, Xu D, Wang W
Journal of cellular physiology 2019 Dec;234(12):22057-22070
Journal of cellular physiology 2019 Dec;234(12):22057-22070
The cellular stress proteins CHCHD10 and MNRR1 (CHCHD2): Partners in mitochondrial and nuclear function and dysfunction.
Purandare N, Somayajulu M, Hüttemann M, Grossman LI, Aras S
The Journal of biological chemistry 2018 Apr 27;293(17):6517-6529
The Journal of biological chemistry 2018 Apr 27;293(17):6517-6529
Control of human energy expenditure by cytochrome c oxidase subunit IV-2.
Schiffer TA, Peleli M, Sundqvist ML, Ekblom B, Lundberg JO, Weitzberg E, Larsen FJ
American journal of physiology. Cell physiology 2016 Sep 1;311(3):C452-61
American journal of physiology. Cell physiology 2016 Sep 1;311(3):C452-61
Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice.
Cloonan SM, Glass K, Laucho-Contreras ME, Bhashyam AR, Cervo M, Pabón MA, Konrad C, Polverino F, Siempos II, Perez E, Mizumura K, Ghosh MC, Parameswaran H, Williams NC, Rooney KT, Chen ZH, Goldklang MP, Yuan GC, Moore SC, Demeo DL, Rouault TA, D'Armiento JM, Schon EA, Manfredi G, Quackenbush J, Mahmood A, Silverman EK, Owen CA, Choi AM
Nature medicine 2016 Feb;22(2):163-74
Nature medicine 2016 Feb;22(2):163-74
Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2.
Aras S, Pak O, Sommer N, Finley R Jr, Hüttemann M, Weissmann N, Grossman LI
Nucleic acids research 2013 Feb 1;41(4):2255-66
Nucleic acids research 2013 Feb 1;41(4):2255-66
Differential regulation of HIF-mediated pathways increases mitochondrial metabolism and ATP production in hypoxic osteoclasts.
Morten KJ, Badder L, Knowles HJ
The Journal of pathology 2013 Apr;229(5):755-64
The Journal of pathology 2013 Apr;229(5):755-64
Cytochrome c oxidase subunit 4 isoform 2-knockout mice show reduced enzyme activity, airway hyporeactivity, and lung pathology.
Hüttemann M, Lee I, Gao X, Pecina P, Pecinova A, Liu J, Aras S, Sommer N, Sanderson TH, Tost M, Neff F, Aguilar-Pimentel JA, Becker L, Naton B, Rathkolb B, Rozman J, Favor J, Hans W, Prehn C, Puk O, Schrewe A, Sun M, Höfler H, Adamski J, Bekeredjian R, Graw J, Adler T, Busch DH, Klingenspor M, Klopstock T, Ollert M, Wolf E, Fuchs H, Gailus-Durner V, Hrabě de Angelis M, Weissmann N, Doan JW, Bassett DJ, Grossman LI
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Sep;26(9):3916-30
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Sep;26(9):3916-30
Sex- and brain region-specific role of cytochrome c oxidase in 1-methyl-4-phenylpyridinium-mediated astrocyte vulnerability.
Sundar Boyalla S, Barbara Victor M, Roemgens A, Beyer C, Arnold S
Journal of neuroscience research 2011 Dec;89(12):2068-82
Journal of neuroscience research 2011 Dec;89(12):2068-82
Brain region specificity of 3-nitropropionic acid-induced vulnerability of neurons involves cytochrome c oxidase.
Singh S, Misiak M, Beyer C, Arnold S
Neurochemistry international 2010 Oct;57(3):297-305
Neurochemistry international 2010 Oct;57(3):297-305
Brain region-specific vulnerability of astrocytes in response to 3-nitropropionic acid is mediated by cytochrome c oxidase isoform expression.
Misiak M, Singh S, Drewlo S, Beyer C, Arnold S
Cell and tissue research 2010 Jul;341(1):83-93
Cell and tissue research 2010 Jul;341(1):83-93
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of COX4I2 expression in transfected 293T cell line by COX4I2 monoclonal antibody (M01), clone 1F2.Lane 1: COX4I2 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Detection limit for recombinant GST tagged COX4I2 is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of COX4I2 transfected lysate using anti-COX4I2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with COX4I2 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: COX IV Isoform 2 Antibody (1F2) [H00084701-M01] - Analysis of monoclonal antibody to COX4I2 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
