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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB5857 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NKX6.1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NKX6.1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human NKX2E, 2.2, 3.1 or recombinant mouse NKX3.1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P78426- Isotype
- IgG
- Antibody clone number
- 631438
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- NKX6.1 in beta TC-6 Mouse Cell Line. NKX6.1 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human NKX6.1 Monoclonal Antibody (Catalog # MAB5857) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
