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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX82513 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX82513, RRID:AB_11165992
- Product name
- SOX4 antibody, N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of SOX4 antibody (N-term) (GTX82513) in A2058 cell line lysates (35μg/lane). SOX4 (arrow) was detected using the purified Pab.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Fluorescent confocal image of Hela cell stained with SOX4 Antibody (N-term)(GTX82513).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). SOX4 immunoreactivity is localized to Mitochondria significantly and Nucleus weakly.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain reacted with SOX4 Antibody (N-term)(GTX82513), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOX4 Antibody (N-term) (GTX82513) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
