Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
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Validation data
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- Product number
- H00023001-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00023001-M03, RRID:AB_10642595
- Product name
- WDFY3 monoclonal antibody (M03), clone 2F12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant WDFY3.
- Antigen sequence
MGTSKEKAKTATLKQALLGHTDTVTCATASLAYHI
IVSGSRDRTCIIWDLNKLSFLTQLRGHRAPVSALC
INELTGDIVSCAGTYIHVWSINGNPIVSVNTFTGR
SQQIICCCMSEMNEWDTQNVIVTGHSDGVVRFWRM
EFLQVPETPAPEPAEVLEMQEDCPEAQIGQEAQDE
DSSDSEADEQSISQDPKDTPSQPSSTSHRPRAASC
RATAAWCTDSGSDDSRRWSDQLSLDEKDGFIFVNY
SEGQTRAHLQGPLSHPHPNPIEVRNYSRLKPGYRW
ERQLVFRSKLTMHTAFDRKDNAHPAEVTALGISKD
HSRILVGDSRGRVFSWSVSDQPGRSAADHWVKDEG
GDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQ
SEIKRLKISSPVRVCQNCYYNLQHERGSEDGPRNC- Isotype
- IgG
- Antibody clone number
- 2F12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Loss of Wdfy3 in mice alters cerebral cortical neurogenesis reflecting aspects of the autism pathology.
Orosco LA, Ross AP, Cates SL, Scott SE, Wu D, Sohn J, Pleasure D, Pleasure SJ, Adamopoulos IE, Zarbalis KS
Nature communications 2014 Sep 8;5:4692
Nature communications 2014 Sep 8;5:4692
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged WDFY3 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol