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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-03332 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-03332, RRID:AB_1522684
- Product name
- Rabbit Polyclonal WDFY3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20 degrees C. Avoid freeze/thaw cycles.
Submitted references Loss of WDFY3 ameliorates severity of serum transfer-induced arthritis independently of autophagy.
Autophagy-linked FYVE containing protein WDFY3 interacts with TRAF6 and modulates RANKL-induced osteoclastogenesis.
T Cell-Independent Mechanisms Associated with Neutrophil Extracellular Trap Formation and Selective Autophagy in IL-17A-Mediated Epidermal Hyperplasia.
Loss of Wdfy3 in mice alters cerebral cortical neurogenesis reflecting aspects of the autism pathology.
ERADication of EDEM1 occurs by selective autophagy and requires deglycosylation by cytoplasmic peptide N-glycanase.
PaCS is a novel cytoplasmic structure containing functional proteasome and inducible by cytokines/trophic factors.
Wu DJ, Adamopoulos IE
Cellular immunology 2017 Jun;316:61-69
Cellular immunology 2017 Jun;316:61-69
Autophagy-linked FYVE containing protein WDFY3 interacts with TRAF6 and modulates RANKL-induced osteoclastogenesis.
Wu DJ, Gu R, Sarin R, Zavodovskaya R, Chen CP, Christiansen BA, Zarbalis KS, Adamopoulos IE
Journal of autoimmunity 2016 Sep;73:73-84
Journal of autoimmunity 2016 Sep;73:73-84
T Cell-Independent Mechanisms Associated with Neutrophil Extracellular Trap Formation and Selective Autophagy in IL-17A-Mediated Epidermal Hyperplasia.
Suzuki E, Maverakis E, Sarin R, Bouchareychas L, Kuchroo VK, Nestle FO, Adamopoulos IE
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4403-4412
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4403-4412
Loss of Wdfy3 in mice alters cerebral cortical neurogenesis reflecting aspects of the autism pathology.
Orosco LA, Ross AP, Cates SL, Scott SE, Wu D, Sohn J, Pleasure D, Pleasure SJ, Adamopoulos IE, Zarbalis KS
Nature communications 2014 Sep 8;5:4692
Nature communications 2014 Sep 8;5:4692
ERADication of EDEM1 occurs by selective autophagy and requires deglycosylation by cytoplasmic peptide N-glycanase.
Park S, Jang I, Zuber C, Lee Y, Cho JW, Matsuo I, Ito Y, Roth J
Histochemistry and cell biology 2014 Aug;142(2):153-69
Histochemistry and cell biology 2014 Aug;142(2):153-69
PaCS is a novel cytoplasmic structure containing functional proteasome and inducible by cytokines/trophic factors.
Sommi P, Necchi V, Vitali A, Montagna D, De Luigi A, Salmona M, Ricci V, Solcia E
PloS one 2013;8(12):e82560
PloS one 2013;8(12):e82560
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WDFY3 Antibody [NBP1-03332] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NBP1-03332 used for WB at 1 mcg/ml (A and B) and used for IP at 3 mcg/mg lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: WDFY3 Antibody [NBP1-03332] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human glioblastoma using 1:200 dilution of WDFY3 antibody (NBP1-03332). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin
