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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-49702 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous level of total FOXS1 protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Identification of novel GLI1 target genes and regulatory circuits in human cancer cells.
Diao Y, Rahman MF, Vyatkin Y, Azatyan A, St Laurent G, Kapranov P, Zaphiropoulos PG
Molecular oncology 2018 Oct;12(10):1718-1734
Molecular oncology 2018 Oct;12(10):1718-1734
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Foxs1 was performed by loading Mouse heart tissue lysate (40µg) on to a 10%+12% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Foxs1 antibody (Product # PA5-49702) at a 1/250 dilution for 10 seconds, followed by Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Foxs1 was performed by loading Mouse heart tissue lysate (40µg) on to a 10%+12% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Foxs1 antibody (Product # PA5-49702) at a 1/250 dilution for 10 seconds, followed by Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Reciprocal proliferation effects of GLI1 and FOXS1; FOXS1 inhibits GLI1 activity. EdU incorporation assay of Rh36 cells (A) and Daoy cells (B) following transfection with siRNA combinations of siControl + siControl, siControl + siGLI1, siControl + siFOXS1, or siGLI1 + siFOXS1. Data from one representative experiment are shown in the histogram. EdU incorporation assay of Daoy cells (C) following transfection/transduction with siControl + Ad-Vector, siControl + Ad-GLI1, siFOXS1 + Ad-Vector, or siFOXS1 + Ad-GLI1. Adenoviruses were added 6 h after siRNA transfection. Data from one representative experiment are shown in the histogram. (D) A schematic diagram of the proposed model for the FOXS1/GLI1 feedback loop. The transcription factor GLI1 positively regulates the expression of the FOXS1 transcription factor, while FOXS1 negatively regulates GLI1. GLI1 promotes cell proliferation, whereas FOXS1 inhibits cell proliferation. (E) FOXS1 expression reduces GLI1 transcriptional activity. HEK293A cells were co-transfected with pcDNA3.1 vector (pcDNA), pGL3 basic luciferase empty vector (pGL3), pCMV-GLI1-flag (pGLI1), or pcDNA3.1-FOXS1 (pFOXS1), together with the reporter plasmid 12xGLIBS and the control plasmid Renilla. Data from one representative experiment are shown. Error bars indicate standard deviation. Statistical significant, ## P < 0.01, compared to pcDNA; ** P < 0.01, compared to pGLI1, calculated by the Student's t -test. (F) Protein-protein interaction of GLI1 a
