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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP1-05972 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-05972, RRID:AB_1555352
- Product name
- Rabbit Polyclonal DCAF4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DCAF4 Antibody [NBP1-05972] - Detection of Human and Mouse WDR21A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WDR21A antibody NBP1-05972 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DCAF4 Antibody [NBP1-05972] - Whole cell lysate from HeLa and 293T cells. Antibodies: Affinity purified rabbit anti-WDR21A antibody. WDR21A was also immunoprecipitated by another rabbit anti-WDR21A antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DCAF4 Antibody [NBP1-05972] - Detection of human WDR21A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WDR21A antibody NBP1-05972 (lot NBP1-05972-2) used for IP at 6 ug per reaction. WDR21A was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05972-1) and another rabbit anti-WDR21A antibody. For blotting immunoprecipitated WDR21A, NBP1-05972 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
