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LearnMA5-24782
antibody from Invitrogen Antibodies
Targeting: SCAF11
CASP11, SFRS2IP, SIP1, SRRP129, SRSF2IP
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-24782 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCAF11 Monoclonal Antibody (17D9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 17D9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-11 in THP-1 (left, untreated; right, treated) cells. The membrane was probed with Caspase-11 monoclonal antibody (Product # MA5-24782) using a dilution of 100 ng/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-11 in mouse B cell tissue. The membrane was probed with Caspase-11 monoclonal antibody (Product # MA5-24782).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-11 in mouse liver tissue. The membrane was probed with Caspase-11 monoclonal antibody (Product # MA5-24782).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-11 in marrow derived macrophages tissue. The membrane was probed with Caspase-11 monoclonal antibody (Product # MA5-24782).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SCAF11 in mouse liver extract. Samples were incubated in SCAF11 monoclonal antibody (Product # MA5-24782) followed by an anti-rat HRP secondary antibody. Separated on a 4-15% gel and transfered to PVDF membrane. Both procaspase 11 and fully cleaved caspase 11 are detected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Caspase-11 in NIH/3T3 cells using Caspase-11 monoclonal antibody (Product # MA5-24782) with a dilution of 1:100, Phalloidin 568 and DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SCAF11 in formalin fixed paraffin embedded tissue section of mouse lung. Samples were incubated in SCAF11 monoclonal antibody (Product # MA5-24782) using a dilution of 1:100. The antibody generated cytoplasmic signal with more intense staining in the bronchiolar epithelium while the staining signal in alveolar cells was relatively low.
