AF7275

antibody from Novus Biologicals

Targeting: ST3GAL2 SIAT4B, ST3GalA.2, ST3GALII

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7275

Provider

Novus Biologicals

Product name

Sheep Polyclonal ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse and rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ST6GALNAC4 and rhST3GAL1 is observed.

Reactivity

Human, Mouse, Rat

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model.

Karhadkar TR, Pilling D, Cox N, Gomer RH
Scientific reports 2017 Nov 8;7(1):15069

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7275) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.