H00006483-M01

antibody from Abnova Corporation

Targeting: ST3GAL2 SIAT4B, ST3GalA.2, ST3GALII

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006483-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006483-M01, RRID:AB_607101

Product name

ST3GAL2 monoclonal antibody (M01), clone 1E12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ST3GAL2.

Antigen sequence

HHSMATLPYLDSGALDGTHRVKLVPGYAGLQRLSK
ERLSGKSCACRRCMGDAGASDWFDSHFDGNISPVW
TRENMDLPPDVQRWWMMLQPQFKSHNTNEV


Isotype

IgG

Antibody clone number

1E12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ST3GAL2 monoclonal antibody (M01), clone 1E12 Western Blot analysis of ST3GAL2 expression in K-562 ( Cat # L009V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ST3GAL2 expression in transfected 293T cell line by ST3GAL2 monoclonal antibody (M01), clone 1E12.Lane 1: ST3GAL2 transfected lysate (Predicted MW: 40.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ST3GAL2 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to ST3GAL2 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 1 ~ 10 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol