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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-112275 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ST3GAL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ST3GAL2 using ST3GAL2 Polyclonal Antibody (Product # PA5-112275) at a dilution of 1:500. Lane A: U-251 MG whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ST3GAL2 was performed on (Lane A) 0.5 mg U251MG whole cell lysate using 1 µL of ST3GAL2 Polyclonal Antibody (Product # PA5-112275) at a dilution of 1:500, and 60 µg of Immunomagnetic beads Protein A/G. A Clean-Blot IP Detection Reagent (HRP) was used as a secondary antibody at a dilution of 1:1,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 45 kDa.
