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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF7275 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse and rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ST6GALNAC4 and rhST3GAL1 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_008858- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model.
Karhadkar TR, Pilling D, Cox N, Gomer RH
Scientific reports 2017 Nov 8;7(1):15069
Scientific reports 2017 Nov 8;7(1):15069
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7275) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
