AF1143

antibody from Novus Biologicals

Targeting: TYMP ECGF1, MNGIE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1143

Provider

Novus Biologicals

Product name

Goat Polyclonal PD-ECGF/Thymidine Phosphorylase Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human PD-ECGF/Thymidine Phosphorylase in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human PD-ECGF/Thymidine Phosphorylase by Western Blot. Detection of Human PD-ECGF/Thymidine Phosphorylase by Western Blot.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

PD-ECGF/Thymidine Phosphorylase in Human Prostate Cancer Tissue. PD-ECGF/Thymidine Phosphorylase was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 5 µg/mL Goat Anti-Human PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1143) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by PD-ECGF/Thymidine Phosphorylase and Neutralization by Human PD-ECGF/Thymidine Phosphorylase Antibody. Recombinant Human PD-ECGF/Thymidine Phosphorylase (Catalog # 229-PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD-ECGF/Thymidine Phosphorylase (150 ng/mL) is neutralized (green line) by increasing concentrations of Human PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1143). The ND₅₀ is typically 0.5-2.0 µg/mL.