AF7568

antibody from R&D Systems

Targeting: TYMP ECGF1, MNGIE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7568

Provider

R&D Systems

Product name

Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects PD-ECGF/Thymidine Phosphorylase in direct ELISAs. Detects human, mouse, and rat PD-ECGF/Thymidine Phosphorylase in Western blots.

Reactivity

Human, Mouse, Rat

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q99N42


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse PD-ECGF/Thymidine Phosphorylase by Western Blot. Western blot shows lysates of L1210 mouse lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

PD-ECGF/Thymidine Phosphorylase in Mouse Embryonic Brain. PD-ECGF/Thymidine Phosphorylase was detected in immersion fixed frozen sections of mouse embryonic brain (13 d.p.c.) using Sheep Anti-Mouse PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7568) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.