MA5-34946

antibody from Invitrogen Antibodies

Targeting: TYMP ECGF1, MNGIE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34946

Provider

Invitrogen Antibodies

Product name

Thymidine Phosphorylase Monoclonal Antibody (A1A10)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A549 cell, human liver carcinoma tissue, human spleen tissue, SiHa cell.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

A1A10

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Thymidine Phosphorylase in A549 lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with Thymidine Phosphorylase monoclonal antibody (Product # MA5-34946), at a dilution of 1:100 (5% BSA, 2 hours), followed by Goat Anti-Mouse IgG - HRP (1 hour) with a dilution of 1:5000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Thymidine Phosphorylase in paraffin-embedded human spleen tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Thymidine Phosphorylase monoclonal antibody (Product # MA5-34946) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Thymidine Phosphorylase in paraffin-embedded human liver carcinoma tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Thymidine Phosphorylase monoclonal antibody (Product # MA5-34946) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Thymidine Phosphorylase in SiHa cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with Thymidine Phosphorylase monoclonal antibody (Product # MA5-34946) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated Goat anti-Mouse IgG with a dilution of 1:1000 (30 min).