H00201161-M05

antibody from Abnova Corporation

Targeting: CENPV CENP-V, p30, PRR6

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00201161-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00201161-M05, RRID:AB_1671594

Product name

CENPV monoclonal antibody (M05), clone 1D9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant CENPV.

Antigen sequence

ICKKKQNRHFIVPASRFKLLKGAEHITTYTFNTHK
AQHTFCKRCGVQSFYTPRSNPGGFGIAPHCLDEGT
VRSMVTEEFNGSDWEKAMKEHKTIKNMSKE


Isotype

IgG

Antibody clone number

1D9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CENPV expression in transfected 293T cell line by CENPV monoclonal antibody (M05), clone 1D9.Lane 1: CENPV transfected lysate (Predicted MW: 29.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CENPV is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to CENPV on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol