PA5-20320

antibody from Invitrogen Antibodies

Targeting: TSLP

Antibody data

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Validation data

Product number: PA5-20320 - Provider product page
Provider: Invitrogen Antibodies
Product name: TSLP Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is A-20 cell lysate.
Concentration: 1 mg/mL

TSLP protein structure - PA5-20320 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of A-20 cell lysate using a TSLP polyclonal antibody (Product # PA5-20320) at (A) 0.5, (B) 1 and (C) 2 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Human Heart (Lane 1) and Human Prostate (Lane 2). Loading: 15 µg of lysates per lane. Antibodies: TSLP Polyclonal Antibody (Product # PA5-20320) (4 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Mouse A-20 Cell Line. Loading: 15 µg of lysates per lane. Antibodies: TSLP Polyclonal Antibody (Product # PA5-20320) (A: 0.5 µg/mL, B: 1 µg/mL, C: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation of TSLP with Human Recombinant protein. Loading: 30 ng of TSLP partial human recombinant protein per lane. Antibodies: TSLP Polyclonal Antibody (Product # PA5-20320) (Lane 1: 0.25 µg/mL, Lane 2: 0.5 µg/mL, Lane 3: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. TLSP partial human recombinant protein: 15 kD, the observed bands at 30 kD and 45 kD are the dimer and trimer, respectively.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Brain Tissue labeling TSLP with TSLP Polyclonal Antibody (Product # PA5-20320) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling TSLP with TSLP Polyclonal Antibody (Product # PA5-20320) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using TSLP Polyclonal Antibody (Product # PA5-20320) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using TSLP Polyclonal Antibody (Product # PA5-20320) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.