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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Blocking/Neutralizing [1]
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- Product number
- AF1398 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TSLP Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TSLP in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse TSLP and recombinant human TSLP R is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q969D9- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Estrogen restricts the apoptosis of endometrial stromal cells by promoting TSLP secretion.
Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance.
TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils.
The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis.
ΔNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis.
Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells.
Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin.
Thymic stromal lymphopoietin is produced by dendritic cells.
Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
Constitutive and inducible thymic stromal lymphopoietin expression in human airway smooth muscle cells: role in chronic obstructive pulmonary disease.
Yang HL, Chang KK, Mei J, Zhou WJ, Liu LB, Yao L, Meng Y, Wang MY, Ha SY, Lai ZZ, Ye JF, Li DJ, Li MQ
Molecular medicine reports 2018 Nov;18(5):4410-4416
Molecular medicine reports 2018 Nov;18(5):4410-4416
Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance.
Vizio B, Boita M, Cristiano C, Mazibrada J, Bosco O, Novarino A, Prati A, Sciascia S, Rolla G, Ciuffreda L, Montrucchio G, Bellone G
Oncotarget 2018 Aug 28;9(67):32795-32809
Oncotarget 2018 Aug 28;9(67):32795-32809
TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils.
Zhang B, Wei CY, Chang KK, Yu JJ, Zhou WJ, Yang HL, Shao J, Yu JJ, Li MQ, Xie F
Oncology letters 2017 Dec;14(6):7483-7488
Oncology letters 2017 Dec;14(6):7483-7488
The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis.
Herro R, Antunes RDS, Aguilera AR, Tamada K, Croft M
The Journal of investigative dermatology 2015 Aug;135(8):2109-2118
The Journal of investigative dermatology 2015 Aug;135(8):2109-2118
ΔNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis.
Kubo T, Kamekura R, Kumagai A, Kawata K, Yamashita K, Mitsuhashi Y, Kojima T, Sugimoto K, Yoneta A, Sumikawa Y, Yamashita T, Sato N, Himi T, Ichimiya S
PloS one 2014;9(8):e105498
PloS one 2014;9(8):e105498
Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells.
Du MR, Guo PF, Piao HL, Wang SC, Sun C, Jin LP, Tao Y, Li YH, Zhang D, Zhu R, Fu Q, Li DJ
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1502-11
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1502-11
Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin.
Schuepbach-Mallepell S, Philippe V, Brüggen MC, Watanabe H, Roques S, Baldeschi C, Gaide O
The Journal of allergy and clinical immunology 2013 Dec;132(6):1348-57
The Journal of allergy and clinical immunology 2013 Dec;132(6):1348-57
Thymic stromal lymphopoietin is produced by dendritic cells.
Kashyap M, Rochman Y, Spolski R, Samsel L, Leonard WJ
Journal of immunology (Baltimore, Md. : 1950) 2011 Aug 1;187(3):1207-11
Journal of immunology (Baltimore, Md. : 1950) 2011 Aug 1;187(3):1207-11
Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
Blood 2010 Sep 23;116(12):2061-9
Blood 2010 Sep 23;116(12):2061-9
A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
Arthritis and rheumatism 2008 Nov;58(11):3510-9
Arthritis and rheumatism 2008 Nov;58(11):3510-9
Constitutive and inducible thymic stromal lymphopoietin expression in human airway smooth muscle cells: role in chronic obstructive pulmonary disease.
Zhang K, Shan L, Rahman MS, Unruh H, Halayko AJ, Gounni AS
American journal of physiology. Lung cellular and molecular physiology 2007 Aug;293(2):L375-82
American journal of physiology. Lung cellular and molecular physiology 2007 Aug;293(2):L375-82
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by TSLP and Neutralization by Human TSLP Antibody. Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 R alpha and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398). The ND50 is typically 0.05-0.25 µg/mL.
