Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB3077 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FABP5/E-FABP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human FABP5/E-FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human FABP1, 2, 3, 4, 6, 7, or 9 is observed.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q01469
- Isotype
- IgG
- Antibody clone number
- 311215
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cigarette smoke inhibits LPS-induced FABP5 expression by preventing c-Jun binding to the FABP5 promoter.
Molecular determinants of retinoic acid sensitivity in pancreatic cancer.
Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1.
Rao D, Perraud AL, Schmitz C, Gally F
PloS one 2017;12(5):e0178021
PloS one 2017;12(5):e0178021
Molecular determinants of retinoic acid sensitivity in pancreatic cancer.
Gupta S, Pramanik D, Mukherjee R, Campbell NR, Elumalai S, de Wilde RF, Hong SM, Goggins MG, De Jesus-Acosta A, Laheru D, Maitra A
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jan 1;18(1):280-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Jan 1;18(1):280-9
Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1.
Liebertz DJ, Lechner MG, Masood R, Sinha UK, Han J, Puri RK, Correa AJ, Epstein AL
Head & neck oncology 2010 Feb 22;2:5
Head & neck oncology 2010 Feb 22;2:5
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human FABP5/E-FABP by Western Blot. Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP5/E-FABP Monoclonal Antibody (Catalog # MAB3077) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP5/ E-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- FABP5 in HUVEC Human Cells. FABP5 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human FABP5 Monoclonal Antibody (Catalog # MAB3077) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-con-jugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.