- MA5-12526 - Invitrogen Antibodies CD1A antibody

Submitted references Immune myopathy with large histiocyte-related myofiber necrosis.

Pestronk A, Sinha N, Alhumayyd Z, Ly C, Schmidt R, Bucelli R
Neurology 2019 Apr 9;92(15):e1763-e1772

Optimization of Single- and Dual-Color Immunofluorescence Protocols for Formalin-Fixed, Paraffin-Embedded Archival Tissues.

Kajimura J, Ito R, Manley NR, Hale LP
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2016 Feb;64(2):112-24

Fibroblastic reticular cell tumor of the breast: A case report and review of the literature.

Li H, Shen P, Liang Y, Zhang F
Experimental and therapeutic medicine 2016 Feb;11(2):561-564

Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study.

Patyka M, Malamud D, Weissman D, Abrams WR, Kurago Z
PloS one 2015;10(7):e0132942

An intraosseous sclerosing odontogenic tumor predominantly composed of epithelial cells: relation to (so-called) sclerosing odontogenic carcinoma and epithelial-rich central odontogenic fibroma.

Tan SH, Yeo JF, Kheem Pang BN, Petersson F
Oral surgery, oral medicine, oral pathology and oral radiology 2014 Oct;118(4):e119-25

Role of Langerhans cells, Ki-67 protein and apoptosis in acquired cholesteatoma: prospective clinical study.

Akdogan V, Yilmaz I, Canpolat T, Ozluoglu LN
The Journal of laryngology and otology 2013 Mar;127(3):252-9

Immunogenicity of DNA vaccines encoding simian immunodeficiency virus antigen targeted to dendritic cells in rhesus macaques.

Tenbusch M, Ignatius R, Nchinda G, Trumpfheller C, Salazar AM, Töpfer K, Sauermann U, Wagner R, Hannaman D, Tenner-Racz K, Racz P, Stahl-Hennig C, Uberla K
PloS one 2012;7(6):e39038

CXCL17 and ICAM2 are associated with a potential anti-tumor immune response in early intraepithelial stages of human pancreatic carcinogenesis.

Hiraoka N, Yamazaki-Itoh R, Ino Y, Mizuguchi Y, Yamada T, Hirohashi S, Kanai Y
Gastroenterology 2011 Jan;140(1):310-21

Biomolecular network reconstruction identifies T-cell homing factors associated with survival in colorectal cancer.

Mlecnik B, Tosolini M, Charoentong P, Kirilovsky A, Bindea G, Berger A, Camus M, Gillard M, Bruneval P, Fridman WH, Pagès F, Trajanoski Z, Galon J
Gastroenterology 2010 Apr;138(4):1429-40

Coordination of intratumoral immune reaction and human colorectal cancer recurrence.

Camus M, Tosolini M, Mlecnik B, Pagès F, Kirilovsky A, Berger A, Costes A, Bindea G, Charoentong P, Bruneval P, Trajanoski Z, Fridman WH, Galon J
Cancer research 2009 Mar 15;69(6):2685-93

Epithelioid smooth muscle tumors of the uterus do not express CD1a: a potential immunohistochemical adjunct in their distinction from uterine perivascular epithelioid cell tumors.

Fadare O, Liang SX
Annals of diagnostic pathology 2008 Dec;12(6):401-5

Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.

Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A
Blood 2008 Dec 1;112(12):4401-10

Tumor-derived TGFbeta-1 induces dendritic cell apoptosis in the sentinel lymph node.

Ito M, Minamiya Y, Kawai H, Saito S, Saito H, Nakagawa T, Imai K, Hirokawa M, Ogawa J
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5637-43

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CD1a was performed by loading 25 µg of Jurkat cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD1a monoclonal antibody (Product # MA5-12526) at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~49 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human tonsil stained with CD1a antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of interdigitating cells of stratified squamous epithelium of tonsil.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD1a in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD1a monoclonal antibody (Product # MA5-12526) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD1a in K562 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD1a monoclonal antibody (Product # MA5-12526) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD1a showing staining in the membrane of NIH-3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD1a monoclonal antibody (Product # MA5-12526) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

MA5-12526

Antibody data