Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-40826 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-40826, RRID:AB_789987
- Product name
- Rabbit Polyclonal Geminin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Polo-like kinase 1 depletion induces DNA damage in early S prior to caspase activation.
Yim H, Erikson RL
Molecular and cellular biology 2009 May;29(10):2609-21
Molecular and cellular biology 2009 May;29(10):2609-21
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Geminin Antibody [NB100-40826] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Geminin antibody NB100-40826 used for IP at 3 mcg/mg lysate. Geminin was also immunoprecipitated by another rabbit anti-Geminin antibodies and NB100-40827, which recognize downstream epitopes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Geminin Antibody [NB100-40826] - Detection of human Geminin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Geminin antibody NB100-40826 used for IP at 3 ug per reaction. Geminin was also immunoprecipitated by rabbit anti-Geminin antibody NB100-40827. For blotting immunoprecipitated Geminin, NB100-40826 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40826] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Geminin used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Geminin Antibody [NB100-40826] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Geminin Antibody [NB100-40826] - Detection of human Geminin by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin (NB100-40826). Detection: DAB
