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- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 14929-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14929-1-AP, RRID:AB_2166123
- Product name
- POFUT1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated POFUT1 antibody (Cat. #14929-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder.
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.
Epiregulin promotes trophoblast epithelial-mesenchymal transition through poFUT1 and O-fucosylation by poFUT1 on uPA.
Progesterone promotes embryo adhesion by upregulating c-Fos/c-Jun transcription factor-mediated poFUT1 expression†.
poFUT1 promotes uterine angiogenesis and vascular remodeling via enhancing the O-fucosylation on uPA.
PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.
POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.
Downregulated protein O-fucosyl transferase 1 (Pofut1) expression exerts antiproliferative and antiadhesive effects on hepatocytes by inhibiting Notch signalling.
LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.
Feichtinger RG, Hüllen A, Koller A, Kotzot D, Grote V, Rapp E, Hofbauer P, Brugger K, Thiel C, Mayr JA, Wortmann SB
EMBO molecular medicine 2021 Sep 7;13(9):e14332
EMBO molecular medicine 2021 Sep 7;13(9):e14332
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.
Huang H, Zhou P, Wei J, Long L, Shi H, Dhungana Y, Chapman NM, Fu G, Saravia J, Raynor JL, Liu S, Palacios G, Wang YD, Qian C, Yu J, Chi H
Cell 2021 Mar 4;184(5):1245-1261.e21
Cell 2021 Mar 4;184(5):1245-1261.e21
Epiregulin promotes trophoblast epithelial-mesenchymal transition through poFUT1 and O-fucosylation by poFUT1 on uPA.
Cui X, Wang H, Li Y, Chen T, Liu S, Yan Q
Cell proliferation 2020 Feb;53(2):e12745
Cell proliferation 2020 Feb;53(2):e12745
Progesterone promotes embryo adhesion by upregulating c-Fos/c-Jun transcription factor-mediated poFUT1 expression†.
Cui X, Sun J, Liang C, Zheng Q, Yang X, Liu S, Yan Q
Biology of reproduction 2019 Oct 25;101(4):675-685
Biology of reproduction 2019 Oct 25;101(4):675-685
poFUT1 promotes uterine angiogenesis and vascular remodeling via enhancing the O-fucosylation on uPA.
Zhang D, Yang Y, Liang C, Liu J, Wang H, Liu S, Yan Q
Cell death & disease 2019 Oct 10;10(10):775
Cell death & disease 2019 Oct 10;10(10):775
PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.
Li D, Lin C, Li N, Du Y, Yang C, Bai Y, Feng Z, Su C, Wu R, Song S, Yan P, Chen M, Jain A, Huang L, Zhang Y, Li X
EBioMedicine 2019 Jul;45:124-138
EBioMedicine 2019 Jul;45:124-138
POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.
Du Y, Li D, Li N, Su C, Yang C, Lin C, Chen M, Wu R, Li X, Hu G
Cell death & disease 2018 Sep 24;9(10):995
Cell death & disease 2018 Sep 24;9(10):995
Downregulated protein O-fucosyl transferase 1 (Pofut1) expression exerts antiproliferative and antiadhesive effects on hepatocytes by inhibiting Notch signalling.
Annani-Akollor ME, Wang S, Fan J, Liu L, Padhiar AA, Zhang J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2014 Jul;68(6):785-90
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2014 Jul;68(6):785-90
LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.
Liu S, Wang J, Qin HM, Yan XM, Yang XS, Liu C, Yan Q
Cell death & disease 2014 Aug 28;5:e1396
Cell death & disease 2014 Aug 28;5:e1396
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 14929-1-AP(POFUT1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells, using POFUT1 antibody 14929-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver cancer using 14929-1-AP(POFUT1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver cancer using 14929-1-AP(POFUT1 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
