Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 14929-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POFUT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGAAAWARP LSVSFLLLLL PLPGMPAGSW DPAGYLLYCP CMGRFGNQAD HFLGSLAFAK LLNRTLAVPP WIEYQHHKPP FTNLHVSYQK YFKLEPLQAY HRVISLEDFM EKLAPTHWPP EKRVAYCFEV AAQRSPDKKT CPMKEGNPFG PFWDQFHVSF NKSELFTGIS FSASYREQWS QRR (1-182 aa encoded by BC000582)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human kidney tissue were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HUVEC cells were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells, using POFUT1 antibody 14929-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver using 14929-1-AP (POFUT1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver using 14929-1-AP (POFUT1 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-POFUT1 (IP:14929-1-AP, 4ug; Detection:14929-1-AP 1:500) with HepG2 cells lysate 2400ug.