EB11528

antibody from Everest Biotech

Targeting: POFUT1 FUT12, KIAA0180, O-Fuc-T, O-FUT

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

EB11528

Provider

Everest Biotech

Product name

Goat anti-POFUT1 Antibody

Antibody type

Polyclonal

Description

Goat anti-POFUT1 Antibody

Reactivity

Human

Host

Goat

Antigen sequence

C-TNLHVSYQKYFKLEP


Epitope

internal region

Isotype

IgG

Vial size

100µg specific antibody in 200µl

Concentration

0.5 mg/ml

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Western blot

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human POFUT1 with C-terminal MYC tag probed with EB11528 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB11528 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB11528 (2µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.