Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-97884 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITGB8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.34 mg/mL
- Storage
- -20°C or -80°C if preferred
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using ITGB8 Polyclonal Antibody (Product # PA5-97884) and a 86 kDa band corresponding to ITGB8 was observed across cell lines except LNCaP along with an uncharacterised band (*) at ~30-40 kDa. Membrane extracts (30 µg lysate) of A-431 (Lane 1), HaCa T (Lane 2) and LNCaP (Lane 3) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1500 (Product # A44115). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ITGB8 was achieved by transfecting A-431 cells with ITGB8 specific siRNAs (Silencer® select Product # s7600, s199507). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the ITGB8 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed ITGB8 Polyclonal Antibody (Product # PA5-97884, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ITGB8.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ITGB8 using a ITGB8 Polyclonal antibody (Product # PA5-97884) at a concentration of 1.52 µg/mL. Lane 1: 293T whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: A549 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 86 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ITGB8 in paraffin embedded human placenta tissue using a ITGB8 polyclonal antibody (Product # PA5-97884) at a dilution of 1:100.