11525-1-AP

antibody from Invitrogen Antibodies

Targeting: MCC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11525-1-AP

Provider

Invitrogen Antibodies

Product name

MCC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AGVGSSPGD QSGDENITQM LKRAHDCRKT AENAAKALLM KLDGSCGGAF AVAGCSVQPW ESLSSNSHTS TTSSTASSCD TEFTKEDEQR LKDYIQQLKN DRAAVKLTML ELESIHIDPL SYDVKPRGDS QRLDLENAVL MQELMAMKEE MAELKAQLYL LEKEKKALEL KLSTREAQEQ AYLVHIEHLK SEVEEQKEQR MRSLSSTSSG SKDKPGKECA DAASPALSLA ELRTTCSENE LAAEFTNAIR REKKLKARVQ ELVSALERLT KSSEIRHQQS AEFVNDLKRA NSNLVAAYEK AKKKHQNKLK KLESQMMAMV ERHETQVRML KQRIALLEEE NSRPHTNETS L (480-829 aa encoded by BC018919)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP ( MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PC-3 cells using 11525-1-AP (MCC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PC-3 cells using 11525-1-AP (MCC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 11525-1-AP (MCC antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MCC (IP:11525-1-AP, 3ug; Detection:11525-1-AP 1:500) with PC-3 cells lysate 5000ug.