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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-97011 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AP1M2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IKYKKNEVFI DVIESVNLLV NANGSVLLSE IVGTIKLKVF LSGMPELRLG LNDRVLFELT GRSKNKSVEL EDVKFHQCVR LSRFDNDRTI SFIPPDGDFE LMSYRLSTQV KPLIWIESVI EKFSHSRVEI MVKAKGQFKK QSVANGVEIS VPVPSDADSP RFKTSVGSAK YVPERNVVIW SIKSFPGGKE YLMRAHFGLP SVEKEEVEGR PPIGVKFEIP YFTVSGIQVR YMKIIEKSGY QALPWVRYIT QSGDYQLRTS; Positive Samples: 22Rv1, BT-474, HepG2, Mouse kidney, Mouse lung, Mouse small intestine; Cellular Location: Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus, Peripheral membrane protein, clathrin-coated vesicle membrane
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.33 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AP1M2 in extracts of various cell lines using AP1M2 Polyclonal Antibody (Product # PA5-97011) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of AP1M2 was performed in U2OS cells using AP1M2 Polyclonal Antibody (Product # PA5-97011) at a dilution of 1:200. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of AP1M2 in paraffin-embedded human liver damage using AP1M2 Polyclonal Antibody (Product # PA5-97011) at a dilution of 1:200.
