15858-1-AP

antibody from Invitrogen Antibodies

Targeting: SF3A1 Prp21, PRPF21, SAP114, SF3a120

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15858-1-AP

Provider

Invitrogen Antibodies

Product name

SF3A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DRSIREKQS DDEVYAPGLD IESSLKQLAE RRTDIFGVEE TAIGKKIGEE EIQKPEEKVT WDGHSGSMAR TQQAAQANIT LQEQIEAIHK AKGLVPEDDT KEKIGPSKPN EIPQQPPPPS SATNIPSSAP PITSVPRPPT MPPPVRTTVV SAVPVMPRPP MASVVRLPPG SVIAPMPPII HAPRINVVPM PPSAPPIMAP RPPPMIVPTA FVPAPPVAPV PAPAPMPPVH PPPPMEDEPT SKKLKTEDSL MPEEEFLRRN KGPVSIKVQV PNMQDKTEWK LNGQVLVFTL PLTDQVSVIK VKIHEATGMP AGKQKLQYEG IFIKDSNSLA YYNMANGAVI HLALKERGGR KK (443-793 aa encoded by BC007684)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HL-60 cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using SF3A1 antibody 15858-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:400 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:400 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SF3A1 (IP:15858-1-AP, 4ug; Detection:15858-1-AP 1:500) with mouse brain tissue lysate 4000ug.