AM20948PU-N

antibody from Acris Antibodies GmbH

Targeting: ADAM17 CD156B, cSVP, TACE

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20948PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM20948PU-N, RRID:AB_10842924

Product name

anti CD156b / ADAM17

Antibody type

Monoclonal

Antigen

Recombinant protein, ADAM17 (NP_003174, 215 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1F6

Vial size

50 µg

Concentration

0.5 mg/mL

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ADAM17 is approximately 3 ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between TGFA and ADAM17. HeLa cells were stained with anti-TGFA rabbit purified polyclonal 1/1200 and anti-ADAM17 mouse monoclonal antibody 1/50.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)