MAB21291

antibody from R&D Systems

Targeting: ADAM17 CD156B, cSVP, TACE

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB21291

Provider

R&D Systems

Product name

Human TACE/ADAM17 Cytosolic Domain Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human TACE/ADAM17 Cytosolic Domain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ADAM33 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P78536


Isotype

IgG

Antibody clone number

136133

Vial size

500 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Nardilysin enhances ectodomain shedding of heparin-binding epidermal growth factor-like growth factor through activation of tumor necrosis factor-alpha-converting enzyme.

Nishi E, Hiraoka Y, Yoshida K, Okawa K, Kita T
The Journal of biological chemistry 2006 Oct 13;281(41):31164-72

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Western Blot Shows Human TACE/ADAM17 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TACE/ADAM17 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TACE/ADAM17 Cytosolic Domain Monoclonal Antibody (Catalog # MAB21291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TACE/ADAM17 at approximately 140 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human TACE/ADAM17 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TACE/ADAM17 Cytosolic Domain Monoclonal Antibody (Catalog # MAB21291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TACE/ADAM17 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Cytosolic Domain Monoclonal Antibody (Catalog # MAB21291, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.