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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-22205 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COMMD7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using COMMD7 Polyclonal Antibody (Product # PA5-22205). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: H1299. 12% SDS PAGE. COMMD7 Polyclonal Antibody (Product # PA5-22205) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COMMD7 Polyclonal Antibody detects COMMD7 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COMMD7 protein stained by COMMD7 Polyclonal Antibody (Product # PA5-22205) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COMMD7 Polyclonal Antibody detects COMMD7 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COMMD7 protein stained by COMMD7 Polyclonal Antibody (Product # PA5-22205) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of COMMD7 was performed in paraffin-embedded mouse lung tissue using COMMD7 Polyclonal Antibody (Product # PA5-22205) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using COMMD7 (Product # PA5-22205) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
