Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-96608 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSUN6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NLPSALVSHV LNPQPGEKIL DLCAAPGGKT THIAALMHDQ GEVIALDKIF NKVEKIKQNA LLLGLNSIRA FCFDGTKAVK LDMVEDTEGE PPFLPESFDR ILLDAPCSGM GQRPNMACTW SVKEVASYQP LQRKLFTAAV QLLKPEGVLV YSTCTITLAE NEEQVAWALT KFPCLQLQPQ EPQIGGEGMR GAGLSCEQLK QLQRFDPSAV PLPDTDMDSL REARREDMLR LANKDSIGFF IAKFVKCKST; Positive Samples: Jurkat, HepG2, HeLa, 293T, Mouse testis
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 3.32 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NSUN6 in extracts of various cell lines using NSUN6 Polyclonal Antibody (Product # PA5-96608) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NSUN6 was performed in 200 µg extracts of HeLa cells using NSUN6 Polyclonal Antibody (Product # PA5-96608). Western blot was performed from the immunoprecipitate using NSUN6 Polyclonal Antibody at a dilution of 1:1000.
