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- Antigen structure
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- Western blot [2]
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- Product number
- PA5-38005 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A recommended positive control is rat liver tissue lysate.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references HNF4α regulates sulfur amino acid metabolism and confers sensitivity to methionine restriction in liver cancer.
Xu Q, Li Y, Gao X, Kang K, Williams JG, Tong L, Liu J, Ji M, Deterding LJ, Tong X, Locasale JW, Li L, Shats I, Li X
Nature communications 2020 Aug 7;11(1):3978
Nature communications 2020 Aug 7;11(1):3978
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDO1/Cysteine Dioxygenase Type 1 in rat liver tissue lysate using a CDO1/Cysteine Dioxygenase Type 1 polyclonal antibody (Product # PA5-38005) at a dilution of 1 µg/mL (A) and 2 µg/mL (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CDO1 in rat liver tissue lysate with CDO1 Polyclonal Antibody (Product # PA5-38005) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Key SAA metabolic enzymes and HNF4alpha are correlated in liver cancers. a SAA metabolic pathways. MAT1A methionine adenosyltransferase 1A, MAT2A methionine adenosyltransferase 2A, MAT2B methionine adenosyltransferase 2B, GNMT glycine N-methyltransferase, SAHH adenosylhomocysteinase, BHMT betaine-homocysteine S-methyltransferase, CBS cystathionine beta-synthase, CTH/CSE cystathionine gamma-lyase, CDO1 cysteine dioxygenase type 1, CSAD cysteine sulfinic acid decarboxylase. Key SAA enzymes investigated in this study are highlighted in orange. b HNF4alpha is clustered together with key SAA metabolic enzymes in liver cancer patients. The mRNA levels of HNF4alpha-regulated liver functional genes (Red), mesenchymal markers (Blue), and key SAA metabolic enzymes (Orange) from 373 liver cancer patients from the TCGA LIHC dataset were clustered and represented by the heatmap as described in Methods. c Expression of key SAA metabolic enzymes is positively correlated with HNF4alpha expression in liver cancer patients ( n = 371). The pair-wise Pearson correlation coefficient and the corresponding p -value between two genes were calculated using MATLAB. Two outlier samples in which HNF4alpha expression levels were more than 3 interquartile ranges (IQRs) below the first quartile among the 373 samples were removed. d Expression of key SAA metabolic enzymes is negatively correlated with TWIST1 expression in liver cancer patients ( n = 373). e HNF4alpha is clustered together with key SA
