- HPA012657 - Atlas Antibodies PLA2R1 antibody

Submitted references Prevalence of Enhanced Granular Expression of Thrombospondin Type-1 Domain-Containing 7A in the Glomeruli of Japanese Patients with Idiopathic Membranous Nephropathy

Iwakura T, Ohashi N, Kato A, Baba S, Yasuda H, Remuzzi G
PLOS ONE 2015 September;10(9)

Membranous Nephropathy Associated With Immunological Disorder-Related Liver Disease: A Retrospective Study of 10 Cases.

Dauvergne M, Moktefi A, Rabant M, Vigneau C, Kofman T, Burtey S, Corpechot C, Stehlé T, Desvaux D, Rioux-Leclercq N, Rouvier P, Knebelmann B, Boffa JJ, Frouget T, Daugas E, Jablonski M, Dahan K, Brocheriou I, Remy P, Grimbert P, Lang P, Chazouilleres O, Sahali D, Audard V
Medicine 2015 Jul;94(30):e1243

Prevalence of anti-phospholipase A2 receptor antibodies in Japanese patients with membranous nephropathy.

Akiyama S, Akiyama M, Imai E, Ozaki T, Matsuo S, Maruyama S
Clinical and experimental nephrology 2015 Aug;19(4):653-60

Kidney biopsy is a sensitive tool for retrospective diagnosis of PLA2R-related membranous nephropathy

Svobodova B, Honsova E, Ronco P, Tesar V, Debiec H
Nephrology Dialysis Transplantation 2013 July;28(7):1839-1844

PLA2R1 kills cancer cells by inducing mitochondrial stress

Augert A, Vindrieux D, Girard C, Le Calvé B, Gras B, Ferrand M, Bouchet B, Puisieux A, de Launoit Y, Simonnet H, Lambeau G, Bernard D
Free Radical Biology and Medicine 2013 December;65

Enhanced expression of the M-type phospholipase A2 receptor in glomeruli correlates with serum receptor antibodies in primary membranous nephropathy

Hoxha E, Kneißler U, Stege G, Zahner G, Thiele I, Panzer U, Harendza S, Helmchen U, Stahl R
Kidney International 2012 October;82(7):797-804

Autoantibodies Specific for the Phospholipase A2 Receptor in Recurrent and De Novo Membranous Nephropathy

Debiec H, Martin L, Jouanneau C, Dautin G, Mesnard L, Rondeau E, Mousson C, Ronco P
American Journal of Transplantation 2011 October;11(10):2144-2152

PLA R Autoantibodies and PLA R Glomerular Deposits in Membranous Nephropathy

Debiec H, Ronco P
New England Journal of Medicine 2011 February;364(7):689-690

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and PLA2R1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423477).

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human kidney and pancreas tissues using HPA012657 antibody. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of normal human kidney shows weak membranous positivity in cells in glomeruli.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney shows weak membranous positivity in cells in glomeruli.
Sample type: HUMAN

HPA012657

Antibody data