H00011059-M01

antibody from Novus Biologicals

Targeting: WWP1 AIP5, DKFZP434D2111

Provider product page for H00011059-M01

Western blot

Antibody data

Antibody Data
Antigen structure
References [14]
Comments [0]
Validations
Western blot [2]
ELISA [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: H00011059-M01 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#H00011059-M01, RRID:AB_549175
Product name: Mouse Monoclonal WWP1 Antibody
Antibody type: Monoclonal
Description: Ascites. WWP1 - WW domain containing E3 ubiquitin protein ligase 1 (1A7)
Reactivity: Human, Simian
Host: Mouse
Isotype: IgG
Vial size: 0.1 mg
Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

WWP1 protein structure - H00011059-M01 shown in red.

Submitted references Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway.

Lee YR, Chen M, Lee JD, Zhang J, Lin SY, Fu TM, Chen H, Ishikawa T, Chiang SY, Katon J, Zhang Y, Shulga YV, Bester AC, Fung J, Monteleone E, Wan L, Shen C, Hsu CH, Papa A, Clohessy JG, Teruya-Feldstein J, Jain S, Wu H, Matesic L, Chen RH, Wei W, Pandolfi PP
Science (New York, N.Y.) 2019 May 17;364(6441)

High expression of WWP1 predicts poor prognosis and associates with tumor progression in human colorectal cancer.

Chen JJ, Zhang W
American journal of cancer research 2018;8(2):256-265

BAP1 promotes breast cancer cell proliferation and metastasis by deubiquitinating KLF5.

Qin J, Zhou Z, Chen W, Wang C, Zhang H, Ge G, Shao M, You D, Fan Z, Xia H, Liu R, Chen C
Nature communications 2015 Sep 30;6:8471

Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis.

Basheer WA, Harris BS, Mentrup HL, Abreha M, Thames EL, Lea JB, Swing DA, Copeland NG, Jenkins NA, Price RL, Matesic LE
Journal of molecular and cellular cardiology 2015 Nov;88:1-13

Ataxin-3 like (ATXN3L), a member of the Josephin family of deubiquitinating enzymes, promotes breast cancer proliferation by deubiquitinating Krüppel-like factor 5 (KLF5).

Ge F, Chen W, Qin J, Zhou Z, Liu R, Liu L, Tan J, Zou T, Li H, Ren G, Chen C
Oncotarget 2015 Aug 28;6(25):21369-78

Functional Characterization of a WWP1/Tiul1 Tumor-derived Mutant Reveals a Paradigm of Its Constitutive Activation in Human Cancer.

Courivaud T, Ferrand N, Elkhattouti A, Kumar S, Levy L, Ferrigno O, Atfi A, Prunier C
The Journal of biological chemistry 2015 Aug 21;290(34):21007-18

WWP2-WWP1 ubiquitin ligase complex coordinated by PPM1G maintains the balance between cellular p73 and ΔNp73 levels.

Chaudhary N, Maddika S
Molecular and cellular biology 2014 Oct 1;34(19):3754-64

Knockdown of WWP1 inhibits growth and induces apoptosis in hepatoma carcinoma cells through the activation of caspase3 and p53.

Cheng Q, Cao X, Yuan F, Li G, Tong T
Biochemical and biophysical research communications 2014 Jun 6;448(3):248-54

WWP1 E3 ligase targets LATS1 for ubiquitin-mediated degradation in breast cancer cells.

Yeung B, Ho KC, Yang X
PloS one 2013;8(4):e61027

Ubiquitin E3 ligase Wwp1 negatively regulates osteoblast function by inhibiting osteoblast differentiation and migration.

Shu L, Zhang H, Boyce BF, Xing L
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2013 Sep;28(9):1925-35

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) delays cellular senescence by promoting p27(Kip1) degradation in human diploid fibroblasts.

Cao X, Xue L, Han L, Ma L, Chen T, Tong T
The Journal of biological chemistry 2011 Sep 23;286(38):33447-56

The E3 ubiquitin ligase WWP1 regulates ΔNp63-dependent transcription through Lys63 linkages.

Peschiaroli A, Scialpi F, Bernassola F, El Sherbini el S, Melino G
Biochemical and biophysical research communications 2010 Nov 12;402(2):425-30

Endogenous spartin (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5.

Edwards TL, Clowes VE, Tsang HT, Connell JW, Sanderson CM, Luzio JP, Reid E
The Biochemical journal 2009 Sep 14;423(1):31-9

Overexpression of WWP1 is associated with the estrogen receptor and insulin-like growth factor receptor 1 in breast carcinoma.

Chen C, Zhou Z, Sheehan CE, Slodkowska E, Sheehan CB, Boguniewicz A, Ross JS
International journal of cancer 2009 Jun 15;124(12):2829-36

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: WWP1 Antibody (1A7) [H00011059-M01] - WWP1 expression in A-431 ( Cat # L015V1 ).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: WWP1 Antibody (1A7) [H00011059-M01] - Analysis of WWP1 expression in transfected 293T cell line by WWP1 monoclonal antibody (M01A), clone 1A7.Lane 1: WWP1 transfected lysate(105.2 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: ELISA: WWP1 Antibody (1A7) [H00011059-M01] - Detection limit for recombinant GST tagged WWP1 is 0.03 ng/ml as a capture antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence: WWP1 Antibody (1A7) [H00011059-M01] - Analysis of monoclonal antibody to WWP1 on A-431 cell. Antibody concentration 10 ug/ml.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoprecipitation: WWP1 Antibody (1A7) [H00011059-M01] - Analysis of WWP1 transfected lysate using anti-WWP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WWP1 MaxPab rabbit polyclonal antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: WWP1 Antibody (1A7) [H00011059-M01] - Analysis of monoclonal antibody to WWP1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.