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LearnMA5-44690
antibody from Invitrogen Antibodies
Targeting: PTPRR
EC-PTP, PCPTP1, PTP-SL, PTPBR7, PTPRQ
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-44690 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCPTP1 Recombinant Rabbit Monoclonal Antibody (JE64-84)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JE64-84
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCPTP1/PTPRQ in Rat brain tissue lysates (20 µg/Lane). Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in PCPTP1/PTPRQ Monoclonal antibody (Product # MA5-44690) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:300,000 for 1 hour at room temperature. Predicted band size: 74 kDa. Observed band size: 74 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PCPTP1/PTPRQ in paraffin-embedded human stomach tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with PCPTP1/PTPRQ Monoclonal antibody (Product # MA5-44690) using a dilution of 1:100 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
