Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4386 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse DMP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse DMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human DMP-1 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q2HJ09
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Midkine-deficiency delays chondrogenesis during the early phase of fracture healing in mice.
Haffner-Luntzer M, Heilmann A, Rapp AE, Beie S, Schinke T, Amling M, Ignatius A, Liedert A
PloS one 2014;9(12):e116282
PloS one 2014;9(12):e116282
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DMP-1 in Embryonic Mouse Rib. DMP-1 was detected in immersion fixed frozen sections of embryonic mouse rib (E15.5) using 10 µg/mL Sheep Anti-Mouse DMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4386) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.