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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA307786 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit monoclonal antibody against IRS-2(clone EPR904(2))
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody against IRS-2(clone EPR904(2))
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- IRS2
- Isotype
- IgG
- Antibody clone number
- EPR904(2)
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - Anti-IRS2 antibody [EPR904(2)]; All lanes : Anti-IRS2 antibody [EPR904(2)] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : 293T + insulin cell lysate.Lane 3 : SH-SY5Y cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 137 kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS2 antibody [EPR904(2)]; Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling IRS2 with TA307786 at 1/50 dilution.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS2 antibody [EPR904(2)]; Immunohistochemical analysis of paraffin-embedded Human muscle tissue labelling IRS2 with TA307786 at 1/50 dilution.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry - Anti-IRS2 antibody; Overlay histogram showing Hela cells stained with TA307786 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in HeLa cells under the same conditions.
- Validation comment
- FC
