Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX81291 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81291, RRID:AB_11170132
- Product name
- IRS2 antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of anti-IRS2 Antibody (C-term) (GTX81291) in 293 cell line lysates (35μg/lane). IRS2 (arrow) was detected using the purified Pab.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Fluorescent image of Hela cell stained with IRS2 Antibody (C-term)(GTX81291).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J).IRS2 immunoreactivity is localized to Cytoplasm significantly.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IRS2 antibody (C-term)(GTX81291), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.