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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- PAB12629 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12629, RRID:AB_10552082
- Product name
- IRS2 (phospho S731) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of IRS2.
- Antigen sequence
ASSPA- Storage
- Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).
Li Y, Soos TJ, Li X, Wu J, Degennaro M, Sun X, Littman DR, Birnbaum MJ, Polakiewicz RD
The Journal of biological chemistry 2004 Oct 29;279(44):45304-7
The Journal of biological chemistry 2004 Oct 29;279(44):45304-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The cell lysate derived from conditioned NIH/3T3 was immunoprecipitated by IRS2 (phospho S731) polyclonal antibody (Cat # PAB12629), then immunoprobed by the same antibody at 1 : 500 (lane 1). Lane 2 is a negative control.
- Validation comment
- Immunoprecipitation
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot Blot : 1 ug peptide was blot onto NC membrane.A : IRS2 (pS731).B : IRS2 (non phosphorylated).C : Non-related Phosphopeptide. Followed by rabbit antibodies incubation at a 1 : 1000 dilution :1. IRS2 (phospho S731) polyclonal antibody (Cat # PAB12629).2. IRS2 polyclonal antibody (Cat # PAB12697).
- Validation comment
- Dot Blot (Peptide)
